Expression of Interest

Contact Person/Scientist in Charge

  • Name and surname: Pilar S. Testillano
  • Email: testillanoSPAMFILTER@cib.csic.es

Centro de Investigaciones Biologicas (CIB-CSIC)

Department / Institute / Centre

  • Name: Centro de Investigaciones Biológicas (CIB-CSIC)
  • Address: Ramiro de Maetzu 9, E-28040 Madrid (Spain)
  • Province: Madrid

Research Area

  • Life Sciences (LIF)

Brief description of the institution:

The CIB is a multidisciplinary research institution, at the forefront of biological research. Initially focusing on biology and biomedicine, sensu stricto, today the CIB is covering a broad spectrum of knowledge and research, including agricultural sciences, chemistry or biophysics.
Using biology as a common denominator, the CIB mission is manifold, but mainly fostering education, research and technology transfer to society. The scientific focus of the institute is mainly driven by some of the major societal challenges as defined by European citizens: health (medicine), food security (agriculture) & environment (ecology).
The CIB is a unique meeting place for scientists covering a broad spectrum of expertise, such as molecular and cellular biologists, geneticists, microbiologists, biophysics, biochemists, working at the heart of the largest university campus in Spain (Complutense University).
 It is one of the more emblematic research institutes of the Spanish National Research Council (CSIC). With a track record of over 60 years, the CIB has been able to attract competitive funding at the local, national and international level to finance its research projects,

Brief description of the Centre/Research Group (including URL if applicable):

 The Centro de Investigaciones Biológicas (Centre for Biological Research) (CIB) is a multidisciplinary research institute, which has been at the forefront of biological research since its creation in 1953. Initially focusing on biology and biomedicine, sensu stricto, today the CIB is covering a broad spectrum of knowledge and research, including agricultural sciences, chemistry or biophysics. The CIB is a unique meeting place for scientists covering a broad spectrum of expertise, such as molecular and cellular biologists, geneticists, microbiologists, biophysics, biochemists, working at the heart of the largest university campus in Spain (Complutense University).
The CIB, with a workforce of over 550 people, is one of the more emblematic research institutes of the Spanish National Research Council (CSIC) and is organised in 5 research departments: Environmental Biology, Chemical and Physical Biology, Cellular and Molecular Medicine, Molecular Microbiology and Infection Biology, Cellular and Molecular Biology (formerly designated as Cell Proliferation and Development).

The research activities are supported by 11 state-of-the-art core facilities, covering all commonly used life science technologies, including genomics and proteomic and imaging platforms.

Project description:

We analyze the regulation of the stress-induced pollen embryogenesis, process in which the microspore is reprogrammed, acquires totipotency and originates an embryo and a doubled-haploid (DH) plant, potent biotechnological tool in plant breeding (through DH technologies) but with limited efficiency in many agrofood and forestry species.

We characterize the determinant factors of cell totipotency acquisition, reprogramming and embryogenesis initiation, in response to stress, for their use as selective targets in strategies for optimization of the system and application in breeding programs of crops, agronomic and environmental. By means of a multidisciplinary and integrated approach with techniques of in situ molecular identification, correlative microscopy, molecular and cell biology and plant physiology, we studied the following elements and processes, which have a key role in the pollen reprogramming: a) Programmed cell death (PCD) and autophagy, which regulate the balance between cell survival and death during the process; b) epigenetic regulation operated by DNA methylation and histone modifications, activators and repressors of transcriptional activity; c) dynamics of phytohormones (auxins and its balance with cytokinins); d) cell wall remodeling mediated by pectin methyl esterase and arabinogalactan proteins (AGPs). We use the model systems of rapeseed and barley, and evaluate the aplicability and extension of the results to other species of interest for agriculture (horticulturals and fruit trees), bioenergy or forestry (cork oak).

Details of the research gorup (funding, members, last publications, etc) can be found at the lab web page: https://www.cib.csic.es/research/microbial-and-plant-biotechnology/pollen-biotechnology-crop-plants

Applications

CV & Motivation letter

Contact details of 2 persons of reference

Deadline: June 15th, 2018

I want to contact the Institution

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